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tslp  (R&D Systems)


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    Structured Review

    R&D Systems tslp
    Tslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tslp+antibody/Human+TSLP+DuoSet+ELISA/10__1016_slash_j__microc__2026__117495-97-64-69
    Average 94 stars, based on 25 article reviews
    tslp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Saline:

    Article Title: Experimental Asthma Persists in IL33 Receptor Knockout Mice Due to the Emergence of a TSLP-driven IL9+ and IL13+ ILC2 subpopulations
    Article Snippet: .. Antibody, pharmacological and genetic interventions For TSLP blockade experiments an anti-TSLP antibody (#MAB555 from R&D, Inc., 20μg/20μL in saline intranasally) was delivered for 3 consecutive days, 3 days prior to analysis in week 10. ..

    Article Title: Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis
    Article Snippet: .. After washing with phosphate-buffered saline without calcium or magnesium, the cells were incubated overnight with an anti-TSLP antibody (R&D Systems, Minneapolis, MN, USA), anti-IL-4 and IL-13 (H-112 and H-129, respectively; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or their isotype-matched control immunoglobulin (Ig)G (Santa Cruz Biotechnology) at 4°C. .. The cells were then incubated with anti-sheep IgG AlexaFluor 488 (Molecular Probes, Eugene, OR, USA) for 2 h at room temperature while being protected from light.

    Article Title: Experimental Asthma Persists in IL33 Receptor Knockout Mice Due to the Emergence of a TSLP-driven IL9+ and IL13+ ILC2 subpopulations
    Article Snippet: .. For TSLP blockade experiments an anti-TSLP antibody (#MAB555 from R&D, Inc., 20μg/20μL in saline intranasally) was delivered for 3 consecutive days, 3 days prior to analysis in week 10. ..

    Control:

    Article Title: Direct Targeting of STAT3 by Xanthatin Suppresses Allergic Airway Inflammation via Inhibition of TSLP Release and NK2 Cell Polarization.
    Article Snippet: .. For experiments with the anti-TSLP antibody, mice were treated (i.n.) with 20 μg anti-TSLP antibody (MAB555R, R&D Systems, Minneapolis, MN, USA) or isotype control IgG2A (MAB006R) 1 h prior to HDM sensitization daily from day 1 to day 3. ..

    Article Title: Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis
    Article Snippet: .. After washing with phosphate-buffered saline without calcium or magnesium, the cells were incubated overnight with an anti-TSLP antibody (R&D Systems, Minneapolis, MN, USA), anti-IL-4 and IL-13 (H-112 and H-129, respectively; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or their isotype-matched control immunoglobulin (Ig)G (Santa Cruz Biotechnology) at 4°C. .. The cells were then incubated with anti-sheep IgG AlexaFluor 488 (Molecular Probes, Eugene, OR, USA) for 2 h at room temperature while being protected from light.

    Article Title: Thymic stromal lymphopoietin induces corticosteroid resistance in natural helper cells during airway inflammation.
    Article Snippet: Anti-Bcl-xL (54H6), anti-phospho-STAT5 (Tyr694, D47E7) and anti-TBP antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). .. Anti-TSLP antibody, anti-IL-7 antibody and goat IgG isotype control antibody were from R&D systems (Minneapolis, MN, USA). .. Biotin-conjugated anti-CD4 (GK1.5), anti-CD8a (53-6.7), anti-CD11c (HL3), antiCD11b (M1/70), anti-CD19 (1D3), anti-NK1.1 (PK136), anti-Gr-1 (RB6-8C5), antiTER119, anti-B220 (RA3-6B2), Pacific blue-conjugated anti-Sca-1 (E13-161), PerCP Cy5.5-conjugated anti-CD25 (PC61), PE-conjugated anti-Thy1.2 (53-2.1), APC Cy7-conjugated anti-streptavidin, anti-IL-2 antibody (S4B6) and Rat IgG2a isotype control antibody were from BD Pharmingen (San Diego, CA, USA).

    Cell Culture:

    Article Title: TSLP protects against liver I/R injury via activation of the PI3K/Akt pathway
    Article Snippet: Mouse TSLP Quantikine ELISA Kit (catalog MTLP00) was from R&D Systems. .. The reagents for animal treatment and cell culture were as follows: rat IgG (azide free) (catalog 6-001-F), anti-TSLP antibody (catalog MAB555), mouse rTSLP (catalog 555-TS-101), and recombinant mouse IGF-1 (catalog 791-MG-050) were from R&D Systems. .. LY294002 (catalog L9908) and bafilomycin A1 (catalog 19-148) were from Sigma-Aldrich.

    Recombinant:

    Article Title: TSLP protects against liver I/R injury via activation of the PI3K/Akt pathway
    Article Snippet: Mouse TSLP Quantikine ELISA Kit (catalog MTLP00) was from R&D Systems. .. The reagents for animal treatment and cell culture were as follows: rat IgG (azide free) (catalog 6-001-F), anti-TSLP antibody (catalog MAB555), mouse rTSLP (catalog 555-TS-101), and recombinant mouse IGF-1 (catalog 791-MG-050) were from R&D Systems. .. LY294002 (catalog L9908) and bafilomycin A1 (catalog 19-148) were from Sigma-Aldrich.

    Incubation:

    Article Title: Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis
    Article Snippet: .. After washing with phosphate-buffered saline without calcium or magnesium, the cells were incubated overnight with an anti-TSLP antibody (R&D Systems, Minneapolis, MN, USA), anti-IL-4 and IL-13 (H-112 and H-129, respectively; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or their isotype-matched control immunoglobulin (Ig)G (Santa Cruz Biotechnology) at 4°C. .. The cells were then incubated with anti-sheep IgG AlexaFluor 488 (Molecular Probes, Eugene, OR, USA) for 2 h at room temperature while being protected from light.

    Article Title: Organic compounds
    Article Snippet: 10 μl of 1×106 cells/ml in assay buffer (RPMI-1640 w/o phenol red, 10% FCS, penicillin 10 Uml−1/streptomycin 10 μgml−1, 1% puromycin) were added to Costar 96-well white plate (Corning). .. 70 μl of assay buffer and 10 μl of anti-TSLP antibody (10×) in assay buffer was added and incubated for 20 min at 37° C. 10 μl of 5 ng/ml TSLP (R&D Systems; 0.5 ng/ml final concentration) in assay buffer was added to give a final assay volume of 100 μl. ..

    Concentration Assay:

    Article Title: Organic compounds
    Article Snippet: 10 μl of 1×106 cells/ml in assay buffer (RPMI-1640 w/o phenol red, 10% FCS, penicillin 10 Uml−1/streptomycin 10 μgml−1, 1% puromycin) were added to Costar 96-well white plate (Corning). .. 70 μl of assay buffer and 10 μl of anti-TSLP antibody (10×) in assay buffer was added and incubated for 20 min at 37° C. 10 μl of 5 ng/ml TSLP (R&D Systems; 0.5 ng/ml final concentration) in assay buffer was added to give a final assay volume of 100 μl. ..



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    Image Search Results


    Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Article Snippet: Anti-TSLP antibody treatment: Anti-TSLP antibody (HY-P990150, MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Neutralization, Comparison, Saline, Staining, Immunohistochemical staining, Expressing, Two Tailed Test, Control